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KMID : 1094719990040040305
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 4 p.305 ~ p.305
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The expression of petunia flavonoid 3′ and 3′5′ hydroxylase genes in potatoes (Solanum tuberosum cv. Jopung)
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Park Yoon-Kyung
Kim Hong-Hee
Cheong Hyeon-Sook
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Abstract
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Flavonoid 3′ (F3′OH) and 3′5′ hydroxylase (F3′5′OH) play a major role in the synthesis of flavonoids. They are involved in the flavonoid modification and the B-ring hydroxylation produces quercetin and myricetin, respectively. We introduced the petunia F3′OH and F3′5′OH genes in potato and expression of these enzyme was confirmed by Southern and Northern blot analyses in these transgenic plants. In the flavonoid, staining experiment, all transgenic plants with petunia F3′OH and F3′5′OH genes were successfully changed with their green color to orange, confirming that quercetin was synthesized in those plants. Especially, the F3′5′OH transgenic potatoes showed the strongest orange color, and it was revealed by capillary electrophoresis that they produce quercetin one and a half times as much as the untransformed potatoes.
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KEYWORD
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flavonoid 3′ hydroxylase, flavonoid 3′5′ hydroxylase, capillary electrophoresis, quercetin biosynthesis
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